JUST FINISH PART 2
Principles of DNA Sequencing:
Exercise 2. The purpose of this exercise is to provide detailed instruction on the practice of DNA
sequencing. You will be provided an actual autoradiograph (an exposed sheet of x-ray film) of a
DNA sequencing gel for analysis. The sequence deduced from the autoradiogram will differ
from a wild type sequence by a single nucleotide(s). Refer to the handout provided on
Blackboard. This difference represents an actual mutation in the DNA molecule. You are
expected to identify the location(s) of the mutated nucleotide(s). You can also work in groups to
read the sequence and compare results to assure accuracy. Make sure you read the handout
because you will see this information again on your midterm. (25 points)
Part 1 does not require a formal lab report. Just give your answers and the results of the cross. For part 2, a type
written lab report is needed. Use the handout to help you put together your report. You MUST have a cover page.
Total = 50 points
Document for Exercise 2.