Easy Multiple choice questions

Which of the following reaction can be the first committed step of glycolsysis?

a) Glucose-6-Phosphate ↔Frucose-6-Phosphate

b) Glucose-6-Phosphate ↔Frucose-1, 6-Bisphosphate

c) Frucose-1, 6-Bisphosphate↔ Phosphoglyceric acid + Dihydroacetic acid.

d) Frucose-6-Phosphate↔Frucose-1, 6-Bisphosphate


2. Which of the following enzyme do not participate in glycolytic pathway?

a) Hexokinaseb) Enolase c) Pyrophsphatase d) Glucokinase


3. Which tissue performs glycolysis as its major energy generation process?

a) Heart muscle. b) Resting thigh muscle. c) Gut muscle. d) Bicep during boxing.


4. The first step of glycolysis, the conversion of Glc to Glc-6-Phosphate is not considered the first committed step of this metabolic pathway. Because Glc-6-Phosphate can follow many different fate other than glycolysis. Which of the following metabolic pathway is one of them?

a) Gluconeogenesis b) Glycogen synthesis c) TCA cycle d) Myoglycolysis.


5. How many ATP is produced in TCA cycle. Note: ATP produced later in ETC from NADP and FADP should not be included here.

a) Oneb) Twoc) Three d) Four


6. Glycolysis and gluconeogenesis shares many of the same reactions. Following are some reactions form glycolysis and gluconeogenesis:

I. Glc-6-phosphate → Fru-6-Phosphate

II. 1,3-Bisphosphoglycerate → 3-Phosphoglycerate.

III. Oxaloacetate → Phosphoenol pyruvate.

IV. 2-Phosphoglycerate →3-Phosphoglycerate.

Which of these are common in both pathway?

a) I, II, III b) I, II, IV c) I, III, IV d) II, III, IV


7. Michaelis-Menten equation is a relation between which variables?

a) Enzyme concentration and catalytic speed.

b) Substrate concentration and Enzyme concentration.

c) Catalytic speed and Substrate concentration.

d) Product concentration and Enzyme affinity.


8. Which interpretation of Michaelis constant Km is always true?

a) It is the substrate concentration for which the catalytic speed is exactly half of maximum speed.

b) It is the catalytic speed of an enzyme when substrate concentration is 1 mol.

c) It is the exact measure of affinity of enzyme towards a certain substrate.

d) Its the substrate concentration for which the catalytic speed is exactly the maximum speed.


9. Which indicates the slope of double-reciprocal plot?

a) Kcat/Vmax b) Km/Vmax c) Kd/Vmax d) Kavg/Vmax.


10. Which is this enzyme have already achieved catalytic perfection?

a) Triose Phosphate Isomerase.

b) DNA plymerase III.

c) Hexokinase.

d) Lysozyme-5-Phosphatase.


11. Which is the main regulator of Lac operon?

a) Cellular ATP concentration.

b) Lactose concentration in the culture.

c) cAMP concentration.

d) Glucose concentration in the culture.


12. How transcription is terminated in trp operon when trp concentration in cell is adequate?

a) Premature transcription termination.

b) Competitive inhibition.

c) Attenuation.

d) Aberration.


13. Which of the following is not a Post translational modification?

a) Polyadenylation. b) Polykitonylation. c) Splicing. d) Codon editing.


14. Which one is only available in Prokaryotic cell?

a) Poly-A tail.

b) Chromosomal organization.

c) Stop codon.

d) Shine-Delgarno sequence.


15. What does ELISA stands for?


16. Which of the following is an use of ELISA?

a) To detect certain antigen in a sample.

b) To detect the rate of phagocytosis.

c) To measure void ratio.

d) To disinfect used lab utensils.


17. Which of the following are part of complement system?

a) C3, C4, C8a b) C4b2a, C5, C6 c) C1pr2, C3, C4 d) C5b, C9, C0


18. Which of the following is not a complement activation pathway?

a) Classical pathway.

b) Alternative pathway.

c) Enzyme-linked pathway.

d) Lectin pathway.


19. In which phase of the bacterial growth secondary metabolites are produced?

a) Stationary phase. b) Lag phase c) Log phase. d) Decline phase.


20. For the maximum biomass production, which phase of bacterial growth should be maximized?

a) Stationary phase. b) Lag phase c) Log phase. d) Decline phase.


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